D. L. Longo, A. Busato, S. Lanzardo, F. Antico, and S. Aime, “Imaging the pH evolution of an acute kidney injury model by means of iopamidol, a MRI-CEST pH-responsive contrast agent,” Magn. Reson. Med., vol. 70, no. 3, pp. 859 – 864, 2013.
D. D. Castelli, E. Terreno, D. Longo, and S. Aime, “Nanoparticle-based chemical exchange saturation transfer (CEST) agents,” NMR Biomed., vol. 26, no. 7, pp. 839 – 849, 2013.
D. D. Castelli, M. C. Caligara, M. Botta, E. Terreno, and S. Aime, “Combined high resolution NMR and H-1 and O-17 relaxometric study sheds light on the solution structure and dynamics of the Lanthanide(III) complexes of HPDO3A,” Inorg. Chem., vol. 52, no. 12, pp. 7130 –7138, 2013.
V. Catanzaro, C. V. Gringeri, V. Menchise, S. Padovan, C. Boffa, W. Dastrù, L. Chaabane, G. Digilio, and S. Aime, “A R2p/R1p ratiometric procedure to assess matrix metalloproteinase-2 activity by magnetic resonance imaging,” Angew. Chem. Int. Edit., vol. 52, no. 14, pp. 3926 – 3930, 2013.
G. Ferrauto, D. D. Castelli, E. Terreno, and S. Aime, “In vivo MRI visualization of different cell populations labeled with PA-RACEST agents,” Magn. Reson. Med., vol. 69, no. 6, pp. 1703 – 1711, 2013.
F. Reineri, A. Viale, S. Ellena, D. Alberti, T. Boi, G.B. Giovenzana, R. Gobetto, S.S. Premkumar, S. Aime, "15N magnetic resonance hyperpolarization via the reaction of parahydrogen with 15N-propargylcholine, " J. Am. Chem. Soc., vol. 134, no. 27, pp. 11146–52, 2012.
D.D. Castelli, W. Dastru, E. Terreno, E. Cittadino, F. Mainini, E. Torres, M. Spadaro, S. Aime, "In vivo MRI multicontrast kinetic analysis of the uptake and intracellular trafficking of paramagnetically labeled liposomes," Journal Of Controlled Release, vol. 144, no. 3, pp. 271-279, 2010.
G. Digilio, V. Menchise, E. Gianolio, V. Catanzaro, C. Carrera, R. Napolitano, F. Fedeli, S. Aime, "Exofacial Protein Thiols as a Route for the Internalization of Gd(III)-Based Complexes for Magnetic Resonance Imaging Cell Labeling, " Journal Of Medicinal Chemistry, vol. 53, no. 13, pp. 4877-4890, 2010.
L. Tei, A. Barge, S.G. Crich, R. Pagliarin, V. Negri, D. Ramella, G. Cravotto, S. Aime, "Target Visualization by MRI Using the Avidin/Biotin Amplification Route: Synthesis and Testing of a Biotin-Gd-DOTA Monoamide Trimer, " Chemistry-A European Journal, vol. 16, no. 27, pp. 8080-8087, 2010.
L. Tei, G. Mazooz, Y. Shellef, R. Avni, K. Vandoorne, A. Barge, V. Kalchenko, M.W. Dewhirst, L. Chaabane, L. Miragoli, D. Longo, M. Neeman, S. Aime, "Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity, " Contrast Media & Molecular Imaging, vol. 5, no. 4, pp. 213-222, 2010.
F.K. Kalman, S. Geninatti-Crich, S. Aime, "Reduction/Dissolution of a beta-MnOOH Nanophase in the Ferritin Cavity To Yield a Highly Sensitive, Biologically Compatible Magnetic Resonance Imaging Agent, " Angewandte Chemie-International Edition, vol. 49, no. 3, pp. 612-615, 2010.
A. Viale, S. Aime, "Current concepts on hyperpolarized molecules in MRI, " Current Opinion In Chemical Biology, vol. 14, no. 1, pp. 90-96, 2010.
C. Grange, S. Geninatti-Crich, G. Esposito, D. Alberti, L. Tei, B. Bussolati, S. Aime, G. Camussi, "Combined Delivery and Magnetic Resonance Imaging of Neural Cell Adhesion Molecule-Targeted Doxorubicin-Containing Liposomes in Experimentally Induced Kaposi's Sarcoma, " Cancer Research, vol. 70, no. 6, pp. 2180-2190, 2010.
E. Terreno, D.D. Castelli, A. Viale, S. Aime, "Challenges for Molecular Magnetic Resonance Imaging, " Chemical Reviews, vol. 110, no. 5, pp. 3019-3042, 2010.
S. Aime, D. DelliCastelli, S. GeninattiCrich, E. Gianolio, E. Terreno, "Pushing the Sensitivity Envelope of Lanthanide-Based Magnetic Resonance Imaging (MRI) Contrast Agents for Molecular Imaging Applications, " Accounts Of Chemical Research, vol. 42, no. 7, pp. 822-831, 2009.
R. Stefania, L. Tei, A. Barge, SG. Crich, I. Szabo, C. Cabella, G. Cravotto, S. Aime, "Tuning Glutamine Binding Modes in Gd-DOTA-Based Probes for an Improved MRI Visualization of Tumor Cells, " Chemistry-A European Journal, vol. 15, no. 1, pp. 76-85, 2009.
Prof. Silvio Aime is the head of the Molecular Imaging Center of the University of Torino. His primary research is focused on the development of new categories of contrast agents for molecular imaging applications.
The main lines of research activities are the following:
• Synthesis and MRI assessment of high-relaxivity Gd-based chelates (including multimeric derivatives and nano-sized assemblies)
• Synthesis and MRI assessment of novel agents based on chemical exchange saturation transfer (CEST)
• Development of MRI-13C-hyperpolarised molecules via the formation of para-hydrogenated substrates
• Imaging reporters responsive to specific physicochemical and biological parameters of the cellular microenvironment (pH, temperature, metabolites' concentration, enzymatic activity)
• Cellular labelling (stem cells, leukocytes, tumour cells, etc.) by the internalisation of paramagnetic metal chelates
• Tumor “targeting” (AA, LDL, Anionic and cationic transporters, Adhesion molecules, Hyperexpressed receptors, fibrin…)
• Targeting thrombi and plaques with suitably functionalised nanocarriers bearing different types of imaging reporters
• Multiple Sclerosis
• Assessment of changes in Vascular Permeability in neo-formed vessels